A simple, selective, precise and accurate colorimetric method of analysis of Anidulafungin in pharmaceutical dosage form was developed and validated. The solvent used was methanol with methyl orange as a colorimetric reagent. Anidulafungin was detected at 464 nm at room temperature. The linear regression analysis data for the linearity plot showed good linear relationship with correlation coefficient value, R2=0.998 in the concentration range 5–50 µg/ml with slope 0.008, intercept -0.021. The method was validated according to the International Conference on Harmonization (ICH) guidelines for linearity, range, accuracy, precision and specificity and applied on bulk powder and pharmaceutical formulations. Anidulafungin was determined in sterile dosage form in range of 99.73% with 0.327 standard deviation. The accuracy of the method was validated by recovery studies and was found to be significant and under specification limits, with % Recovery 99–101.1 (within acceptable range (98–102%).
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